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How to Minimize Bumping and Foaming

By Harmen Zijlstra, 26 June 2017

It frequently happens that samples being evaporated on the rotavap start to foam or bump. This is highly undesirable as it leads to sample loss and possible contamination. Why does a sample bump and/or foam and what can you do to prevent or at least minimize it?

Bumping occurs when a sample boils rapidly, forming bubbles that cause the sample being concentrated to splash out of the flask. This is normally caused by heating too quick or applying too strong of a vacuum. The chances of it causing damage and splashes into the rotovap are increased if your evaporation flask is more than half full. Go slow, start with little vacuum and visually inspect your sample while decreasing the pressure so that bumping doesn’t occur. The same goes for the water bath temperature, don’t start too hot and keep monitoring the sample while increasing the bath temperature. 

While bumping can often be prevented with great care foaming is much harder to completely avoid. Foaming occurs due to surfactants being present in your sample and can be minimized by slowly applying vacuum, visually monitoring the sample, and opening and closing the stop cock to release the vacuum whenever foam appears. 

There are a few things you can do to help you deal with bumping and foaming issues that may occur despite precautions. Anti-foam agents can be added to prevent foaming and a larger flask can be used to ensure that the foam stays in your evaporation flask. For bumping you can add agents, such as boiling chips, which make the boiling more uniform.

To prevent contamination and sample loss from bumping or foaming, a bump trap or a foam brake can be installed. This secondary flask will collect your sample if it bumps or foams over, avoiding product loss and a contaminated rotovap. Nowadays, more advanced rotovaps such as the RV Control V Auto Rotary Evaporator from IKA and Hei-VAP Precision by Heidolph are equipped with a sensor that will monitor the vapor pressure and slowly adjust the vacuum for you, automatically taking care of bumping and foaming without extra effort on your part.

RV Control V Auto Rotary Evaporator and foam brake from IKA

 

If you're more of the visual type, here's a short video from Benchfly on preventing bumping and mitigating the problems it causes: